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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: ACS Chem Biol. 2012 Jun 26;7(9):1509–1514. doi: 10.1021/cb300241v

Figure 2. Sialidase assay accurately characterizes specificity of bacterial sialidases.

Figure 2

Biotinylated Neu5Acα2-3Lac and Neu5Acα2-6Lac were incubated with Arthrobacter ureafaciens sialidase A (0, 0.55, 1.66, or 5 mU, bars left to right) or Salmonella typhimurium sialidase T (0, 6.4, 19.3, or 58 μU, bars left to right) for 2 h at 37 °C. The PAL method was used to determine the fraction of sialylated glycan remaining after sialidase treatment. Error bars represent standard deviation of three trials.