Biotinylated Neu5Acα2-3Lac and Neu5Acα2-6Lac were incubated with Arthrobacter ureafaciens sialidase A (0, 0.55, 1.66, or 5 mU, bars left to right) or Salmonella typhimurium sialidase T (0, 6.4, 19.3, or 58 μU, bars left to right) for 2 h at 37 °C. The PAL method was used to determine the fraction of sialylated glycan remaining after sialidase treatment. Error bars represent standard deviation of three trials.