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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2012 Aug 29;189(7):3404–3410. doi: 10.4049/jimmunol.1201138

Figure 2. Cytokine release and Notch ligand expression on BMDCs after treatment with LPS or HSP65.

Figure 2

BMDCs from naive mice were incubated with LPS (1 µg/ml), HSP65 (1 or 10 µg/ml), or PBS as a vehicle for 24 hours. Culture supernates were recovered to measure cytokine levels and mRNA was isolated from cells. (A) Cytokine levels. To compensate for the variability in cytokine release by cultured BMDCs from experiment to experiment, the results of cytokine assays were expressed as a percent of the cytokine levels detected after LPS stimulation. (B) Notch ligand expression. The relative expression levels of Notch ligands (Delta1 or Jagged1) were determined by quantitative real-time PCR. cDNA contents were normalized to levels of GAPDH. Results are from three independent experiments and the results for each group are expressed as means±SEM. *p<0.05 compared to vehicle-treated BMDCs.