TABLE 1.
Proteins that co-immunoprecipitate with traceable PKC isoforms.
| A. | ||||
|---|---|---|---|---|
| Protein associated with: |
||||
| Co-immunoprecipitating Protein | PKC-α | score | PKC-δ | score |
| *Cdc42 effector protein-4 (CEP4) | 43 kDa | 193 | 43 kDa | 196 |
| β-Actin | 43 kDa | 1060 | 43 kDa | 906 |
| MAPKK1 | 43 kDa | 53 | ||
| Dynein-1 | 53 kDa | 133 | ||
| LIM protein 3 | 53 kDa | 38 | ||
| *p21-activated kinase-2 (PAK2) | 62 kDa | 58 | ||
| Ras GTPase activating protein-binding protein 1/2 | 62 kDa | 84/103 | ||
| MAPKKK7-interacting protein | 62 kDa | 303 | ||
| AMP-activated protein kinase | 62 kDa | 47 | ||
| Desmoglein | 62 kDa | 118 | 43 kDa | 76 |
| *CLIP-associated protein-1 (CLASP-1) | 200 kDa | 81 | ||
| *Rho-activated kinase-1 and 2 (ROCK-1/2) | 200 kDa | 823/114 | 200 kDa | 868 |
| ARFGAP with Rho-GAP domain (ARAP2) | 200 kDa | 66 | ||
| *Rho/cdc42/Rac activating protein-1 (RICS) | 200 kDa | 51 | 200 kDa | 49 |
| Brefeldin A-inhibitor of GTP-exchange protein-2 (ARFGEF2) | 200 kDa | 318 | ||
| B. | |||||||
|---|---|---|---|---|---|---|---|
| Protein associated with: |
|||||||
| Ref. | PKC-α | score | PKC-δ | score | PKC-ζ | score | |
| VASP (Ser-157) | 41 | 43 kDa | 271 | 43 kDa | 275 | ||
| α-Tubulin (Ser-165) | 23 | 53 kDa | 65 | ||||
| PACSIN 2 | 42 | 62 kDa | 84 | ||||
| EF-1α | 26 | 130 kDa | 57 | ||||
| IQGAP-1 (Ser-1443) | 40 | 200 kDa | 64 | 200 kDa | 249 | ||
| Plectin | 33 | 200 kDa | 3022 | 200 kDa | 37 | ||
| MAP4 (Ser-815) | 43 | 200 kDa | 60 | ||||
A. Proteins were identified by MS/MS, as described in the ‘Methods’. The Mascot protein score is indicated next to each detected protein. Those proteins that contain one or more potential PKC phosphorylation sites at either partial or full consensus sequences are signified by (*).
B Proteins identified by MS/MS that had been previously identified as either a substrate or binding protein of PKC, are shown along with the site of PKC phosphorylation (given in parentheses).