Table 2.
TpTSCO2−/TpT ratio found in (a) the SP repair reaction mediated by a pre-reduced C141A mutant (10 μM) supplemented by varying amount of dithionite or DTT, and (b) photoreaction of TpTSPh (1 mM) supplemented by varying amount of dithionite.
| Dithionite (mM) | 0.02 | 0.05 | 0.2 | 1 | 10 | 10mM DTT |
| TpTSO2−/TpT a | 7.1±1 | 5.8±0.6 | 3.2±0.3 | 2.8±0.3 | 1.8±0.3 | <0.1 |
| TpTSO2−/TpT b | 0.08 ± 0.01 | 0.14 ± 0.01 | 0.18 ± 0.02 | 0.21 ± 0.02 | 0.44 ± 0.03 | 0.22 ± 0.02 c |
b
The yield of the TpTSPh photoreaction is ~ 7%, making the concentration of the TpT radical generated from the photoreaction to be < 7 μM. This concentration should be comparable to the TpT radical concentration produced from the SPL C141A mutant reaction.
c
The reaction was conducted in the presence of both 10 mM dithionite and 10 mM DTT.