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Effect of heparin and EDTA on CPH-nitroxide formation catalyzed by ceruloplasmin in samples prepared with Chelex-treated phosphate buffer. Representative EPR spectra of samples containing (A) 1 mM CPH and 1 µM ceruloplasmin, and in the presence of (B) Heparin (50 U/mL) or (C) EDTA (200 µM) are shown. Control samples in the absence of ceruloplasmin (D, E and F) are shown.
