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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2012 Jul 21;53(7):1514–1521. doi: 10.1016/j.freeradbiomed.2012.07.013

Figure 4.

Figure 4

Effect of transition metal ion chelators in the CPH-nitroxide formation catalyzed by ceruloplasmin and desalted ceruloplasmin in samples prepared with Chelex-treated phosphate buffer. Spectra were taken for reaction mixtures containing CPH (1 mM) and ceruloplasmin (2 µM) in the presence of DTPA (200 µM) or bathocuproine (200 µM). The areas of the first EPR line were normalized to the area of the sample containing only 1 mM CPH. Statistically significant differences compared to the sample of only CPH are shown (* P < 0.05).