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Effect of ceruloplasmin and DTPA in the CPH-nitroxide formation in Chelex-treated phosphate buffer or rat serum. In (A) reaction mixtures were composed of 1 µM ceruloplasmin and1 mM CPH prepared with Chelex-treated phosphate buffer or rat serum, with and without DTPA (200 µM). In (B) samples were prepared as in (A) but without ceruloplasmin. The spectra were recorded after incubation for 10 min at 25°C.
