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. 2012 Aug;166(8):2243–2262. doi: 10.1111/j.1476-5381.2012.01929.x

Figure 6.

Figure 6

CSE up-regulates mRNA expression of NADPH oxidases NOX1, NOX2 and DUOX2 in differentiated human bronchial epithelial cells (D-BECs) that was reversed by roflumilast N-oxide (RNO). D-BECs were pre-incubated with RNO at 2 nM or 1 µM, or dibutyryl cAMP at 1 mM (cAMP), or apocynin (ACY) at 100 µM, or NAC at 1 mM, or vehicle for 30 min and then exposed to CSE (10%) for 24 h. mRNA transcripts of NOX1, NOX2 and DUOX2 were quantified by real-time quantitative RT-PCR as described in Methods. Results are given as means ± SEM of cultures from two donors and three experiments per donor. *P < 0.05 versus control, #P < 0.05 versus CSE. CTR, control.