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. 2012 Aug;166(8):2402–2416. doi: 10.1111/j.1476-5381.2012.01956.x

Figure 6.

Figure 6

Effect of various pH buffers on the currents induced by 30 µM GABA in DH neurones. (A) Average amplitude of the current induced by 30 µM of GABA in presence of 20 mM of the tested buffer plus 1 mM HEPES, normalized to the current recorded in presence of 1 mM HEPES alone. PIPES, HEPES, TRIS, MOPS and imidazole (Imidaz) significantly inhibited the currents induced by 30 µM GABA. MES had no significant effect. (B) Extent of the inhibition mediated by the buffers tested related to the inhibition caused by 20 mM HEPES. The inhibition mediated by PIPES was similar to that mediated by HEPES. Imidazole-, MOPS- and TRIS-mediated inhibitions were significantly weaker than HEPES-mediated inhibition. Only MES, HEPES and PIPES correspond to buffers selected by Good et al. (1966). All experiments were performed at pH 7.3. Numbers within the histogram bars represent the number of neurones recorded. Data represent the mean of normalized values ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ratio values significantly different from unity; n.s., not significant; paired Student's t-test.