Fig. 2.

Stimulatory effect of Cat-S on human ENaC is concentration dependent. Oocytes expressing human αβγ ENaC were incubated for 30 min in protease-free solution (control), in solutions containing different concentrations of Cat-S (0.01, 0.03, 0.1, 0.3, 1, and 3 μM) or in a solution containing chymotrypsin (2 μg/ml). Amiloride-sensitive whole-cell currents (ΔI _ami) were detected before (ΔI _ami initial) and after incubation (ΔI _ami 30 min). Columns represent the relative stimulatory effect on ΔI _ami calculated as the ratio of ΔI _ami 30 min/ΔI _ami initial. Numbers inside the columns indicate the number of individual oocytes measured. N indicates the number of different batches of oocytes