FIG. 10.

Outline of redox proteomics approach. The identification of oxidatively modified protein involves first the separation of proteins by isoelectric point (IEF) followed by the separation of proteins based on relative mobility (Mr). The separation of the proteins is followed by transferring the proteins onto nitrocellulose or polyvinylidene fluoride membrane, probing with the antibody of interest, and determination of oxidatively modified protein by image analysis. Once a protein is identified as oxidatively modified, the protein spot will be excised from the gel, digested with trypsin, and subjected to mass spectrometry for correct identification of the proteins. (To see this illustration in color the reader is referred to the web version of this article at www.liebertonline.com/ars.)