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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Gastroenterology. 2011 Jul 8;141(4):1371–1380.e2. doi: 10.1053/j.gastro.2011.06.071

Figure 5.

Figure 5

PTK6 regulates STAT3 activation in human and mouse colon cell lines. (A) Immunoblot analysis of lysates prepared from HCT116 cell lines containing empty vector or two different PTK6 shRNA’s (shRNA49, shRNA52. (B) The ratio of P-STAT3 over total STAT3 is represented as means +/− standard deviation (*P = 0.002). (C) HCT116 cells expressing empty vector, shRNA49 or shRNA52 were serum starved and stimulated with EGF for 2, 5 or 10 minutes. Immunoblotting was performed using antibodies against PTK6, P-STAT3, total STAT3, and β-actin. (D) The ratio of P-STAT3 over the total STAT3 band density following EGF treatment was quantified using NIH ImageJ. (E) Immunoblot analysis of lysates from wild type YAMC cells and Ptk6 −/− YAMC cells. (F) The ratio of P-STAT3 over the total STAT3 is shown.