Table 1.
Characteristics of agr-Defective Staphylococcus aureus Strains Recovered From Patients
| Genotype |
Blood |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Group | Patient No. | spa Type | spa Motif | CCa | Reference Sequence | agr Group | Strain No. | δ-Hemolysin | agr Mutationb | Predicted Result |
| 1 | 16 | 4 | YHFGFMBQBLO | 8 | NCTC8325 | 1 | 4 | − | 126-IS256 | Frameshift-truncated AgrC |
| 1 | 24 | 21 | UJGBBGGJAGJ | 15 | D61 | 2 | 13 | − | g517a | Ala>Thr at aa 173 in AgrAc |
| 1 | 30 | 532 | WGKAKAOM | 30 | MRSA252 | 3 | 19 | − | No mutation found | None |
| 1 | 34 | 576 | ZDGMDMGMMM | 101 | Not266 | 1 | 26 | − | a409 (−1 bp), g52a | Frameshift-truncated AgrC, Ala>Thr at aa 18 in AgrAd |
| 1 | 46 | 2 | TJMBMDMGMK | 5 | JH1 | 2 | 46 | Weak | No mutation found | None |
| 1 | 53 | 363 | YGFMBQBLO | 8 | NCTC8325 | 1 | 54 | − | g1133a | Gly>Asp at aa 378 in AgrCe |
| 1 | 60 | 385 | TO2MBMDMGMK | 5 | JH1 | 2 | 61 | − | c136t | Gln>Stop at aa 46 in AgrA |
| 1 | 67 | 852 | UJGJAGJ | 15 | D61 | 2 | 78 | − | c771t, c583t | Pro>Pro at aa 316 in AgrC, Arg>Cys at aa 195 in AgrAc |
| 1 | 73 | 33 | WGKAKAOMQQ | 30 | MRSA252 | 3 | 85 | − | t224a | Ile>Asn at aa 75 in AgrAe |
| 1 | 77 | 755 | UJGPLM | 12 | C126 | 2 | 91 | − | t313 (+1 bp), a662 g | Frameshift-truncated AgrC, Tyr>Cys at aa 221 in AgrAc |
| 1 | 84 | 1 | YHGFMBQBLO | 8 | NCTC8325 | 1 | 99 | + | … | … |
| 1 | 110 | 33 | WGKAKAOMQQ | 30 | MRSA252 | 3 | 130 | − | t485c | Phe>Ser at aa 162 in AgrCe |
| 1 | 117 | 44 | WGMQ | 30 | MRSA252 | 3 | 143 | − | 955–985 (−30 bp) | Frameshift-truncated AgrC |
| 1 | 119 | 16 | WGKAKAOMQQQ | 30 | MRSA252 | 3 | 146 | + | … | … |
| 1 | 120 | 664 | YC2BQBLO | 8 | NCTC8325 | 1 | 147 | − | a86c | Asp>Ala at aa 29 in AgrAe |
| 1 | 123 | 1365 | ZFFGU2DMGGM | 25 | D302 | 1 | 150 | + | … | … |
| 1 | 135 | 53 | YGFMBQBLQBLPO | 8 | NCTC8325 | 1 | 165 | − | 121-IS256 | Frameshift- truncated AgrC |
| 1 | 136 | 3 | WGKAOMQ | 8 | NCTC8325 | 1 | 166 | + | … | … |
| 1 | 140 | 184 | ZFGU2DMGGM | 25 | D302 | 1 | 171 | − | t349 (+1 bp), g543t | Frameshift-truncated AgrC, Gln>His at aa 181 in AgrCc |
| 1 | 146 | 385 | TO2MBMDMGMK | 5 | JH1 | 2 | 180 | − | g739a | Glu>Lys at aa 247 in AgrCc |
| 2 | 5 | 1405 | UJJKBPE | 30 | MW2 | 3 | 14 | − | c254a, a514g | Thr>Asn at aa 85 AgrCc, Ile>Val at aa 172 AgrAc |
| 2 | 7 | 43 | WGKAKAOMQ | 30 | MRSA252 | 3 | 18 | − | 667–677 (−11 bp) | Frameshift-truncated AgrC |
| Nasal |
Foci |
|||||||||
| Group | Patient No. | Strain No. | δ-Hemolysin | agr Mutationb | Predicted Result | Strain No. | Specimen Source | δ-Hemolysin | agr Mutationb | Predicted Result |
| 1 | 16 | 13 | − | 126-IS256 | Frameshift-truncated AgrC | … | … | … | … | … |
| 1 | 24 | 39 | − | t826 (−1 bp) | Frameshift-truncated AgrC | … | … | … | … | … |
| 1 | 30 | 54 | − | No mutation found | None | … | … | … | … | … |
| 1 | 34 | 75 | − | a409 (−1 bp), g52a | Frameshift-truncated AgrC, Ala>Thr at aa 18 in AgrAd | … | … | … | … | … |
| 1 | 46 | 138 | − | g1177t | Gly>Cys at 393 aa in AgrCe | … | … | … | … | … |
| 1 | 53 | 164 | + | … | … | … | … | … | … | … |
| 1 | 60 | 191 | + | … | … | … | … | … | … | … |
| 1 | 67 | 253 | − | c771t, c583t | Pro>Pro at aa 316 in AgrC, Arg>Cys at aa 195 in AgrAc | … | … | … | … | … |
| 1 | 73 | 277 | + | … | … | … | … | … | … | … |
| 1 | 77 | 295 | − | t313 (+1 bp), a662 g | Frameshift-truncated AgrC, Tyr>Cys at aa 221 in AgrAc | 296 | Catheter | − | t313 (+1 bp), a662 g | Frameshift-truncated AgrC, Tyr>Cys at aa 221 in AgrAc |
| 1 | 84 | 326 | − | t1107 (+1 bp) | Frameshift-truncated AgrC | … | … | … | … | … |
| 1 | 110 | 430 | + | … | … | … | … | … | … | … |
| 1 | 117 | 466 | + | … | … | 468 | CSF | + | … | … |
| 1 | 119 | 479 | − | t47a | Lys>Met at aa 16 in AgrA | … | … | … | … | … |
| 1 | 120 | 484 | − | a86c | Asp>Ala at aa 29 in AgrAe | … | … | … | … | … |
| 1 | 123 | 494 | − | t2c | Val>Ala at aa 1 in AgrCe | … | … | … | … | … |
| 1 | 135 | 545 | + | … | … | 546 | Wound | − | 121-IS256 | Frameshift-truncated AgrC |
| 1 | 136 | 549 | − | 78–84 (−7 bp) | Frameshift-truncated AgrA | 550 | Catheter | + | … | … |
| 1 | 140 | 564 | − | t349 (+1 bp), g543t | Frameshift-truncated AgrC, Gln>His at aa 181 in AgrCc | 566 | Catheter | − | t349 (+1 bp), g543t | Frameshift-truncated AgrC, Gln>His at aa 181 in AgrCc |
| 1 | 146 | 595 | − | g739a | Glu>Lys at aa 247 in AgrCc | … | … | … | … | … |
| 2 | 5 | 13 | − | c254a, a514 g | Thr>Asn at aa 85 AgrCc, Ile>Val at aa 172 AgrAc | … | … | … | … | … |
| 2 | 7 | 17 | − | 667–677 (−11 bp)g | Frameshift-truncated AgrC | … | … | … | … | … |
Group 1 indicates retrospectively collected strain pairs from patients with bacteremia, and group 2 (in bold) indicates prospectively collected strain pairs from patients who subsequently developed bacteremia (see text).
Abbreviations: −, negative, +, positive; aa, amino acid; bp, base pair; CSF, cerebral spinal fluid.
a spa-type deduced clonal complex (CC).
b Designation corresponds to the region in agrA or agrC of the genome sequenced isolate of the appropriate agr group (Materials and Methods).
c Tolerated by the sorting intolerant from tolerant (SIFT) algorithm.
d Insignificant polymorphism found in closely related agr-positive clone.
e Not tolerated by the SIFT algorithm.
f Not tolerated by the SIFT algorithm (low confidence prediction).
g The indicated mutation was identified in a subpopulation of the culture, which contained additional agr-defective species.