Table 2.

Characteristics of Clonal Complex (CC) 30 Blood Isolates

Patient No.	Strain No.	spa Type	spa Motif	CCa	δ-Hemolysin	agr Mutationb	Predicted Result
167	211	43	WGKAKAOMQ	30	+	No mutation found	None
70	81	33	WGKAKAOMQQ	30	+	No mutation found	None
119	146	16	WGKAKAOMQQQ	30	+	No mutation found	None
56	57	19	XKAKAOMQ	30	+	No mutation found	None
122	149	1384	WGKAKAOLOMQQ	30	Weak	g272a	Ser>Asn at aa 91 AgrCc
163	204	43	WGKAKAOMQ	30	Weak	t487g	Ser>Ala at aa 163 AgrCd
33	22	43	WGKAKAOMQ	30	Weak	No mutation found	None
80	95	43	WGKAKAOMQ	30	Weak	No mutation found	None
89	109	43	WGKAKAOMQ	30	Weak	No mutation found	None
92	105	43	WGKAKAOMQ	30	Weak	No mutation found	None
15	3	33	WGKAKAOMQQ	30	Weak	No mutation found	None
72	84	33	WGKAKAOMQQ	30	Weak	No mutation found	None
75	88	33	WGKAKAOMQQ	30	Weak	No mutation found	None
87	102	33	WGKAKAOMQQ	30	Weak	No mutation found	None
94	112	33	WGKAKAOMQQ	30	Weak	No mutation found	None
103	122	33	WGKAKAOMQQ	30	Weak	No mutation found	None
109	129	33	WGKAKAOMQQ	30	Weak	No mutation found	None
118	144	33	WGKAKAOMQQ	30	Weak	No mutation found	None
131	159	33	WGKAKAOMQQ	30	Weak	No mutation found	None
154	190	33	WGKAKAOMQQ	30	Weak	No mutation found	None
40	40	16	WGKAKAOMQQQ	30	Weak	No mutation found	None
96	114	16	WGKAKAOMQQQ	30	Weak	No mutation found	None
157	194	19	XKAKAOMQ	30	Weak	No mutation found	None
30	19	532	WGKAKAOM	30	−	No mutation found	None
73	85	33	WGKAKAOMQQ	30	−	t224a	Ile>Asn at aa 75 in AgrAc
110	130	33	WGKAKAOMQQ	30	−	t485c	Phe>Ser at aa 162 in AgrCc

Abbreviations: −, negative; +, positive; aa, amino acid.

^a spa-type-deduced CC. Primary grouping of strains is by agr functionality, followed by polymorphism of spa.

^b Designation corresponds to the region in agrC or agrA of genome-sequenced CC30 prototype strain MRSA252, which has an AgrC G55R amino acid change that attenuates agr function (see text).

^c Not tolerated by the sorting intolerant from tolerant (SIFT) algorithm.

^d Tolerated by the SIFT algorithm.