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. Author manuscript; available in PMC: 2013 Nov 2.
Published in final edited form as: Oncogene. 2012 Jun 18;32(18):2304–2314. doi: 10.1038/onc.2012.248

Figure 1. The period of CGNP proliferation is extended in Bax−/− mice.

Figure 1

A,B) IHC for proliferation marker Ki67 (red) at P16 on representative cerebella of Bax+/+ (A) and Bax−/−. (B) littermates demonstrates increased proliferation of Bax-deficient CGNPs when neurogenesis typically wanes. Nuclei are counterstained blue with DAPI. Scale bars represent 500 μm. C) Representative images demonstrating incorporation of thymidine analogue EdU, 24 hours after IP injection at P15 into Bax+/+ and Bax−/− littermates. EdU was visualized by Click-It chemistry with Alexa-488 and EdU+ cells were counted in equivalent regions of EGL in anatomically matched sections from 4 replicate mice of each genotype. Scale bars represent 25 μm D) As an alternative method of quantifying proliferation, cerebella from 3 Bax+/+ and 3 Bax−/− mice were dissociated 24 hours after EdU injection IP at P15 and EdU+ cells counted by FACS. Data presented in the graph are mean proportion of EdU+ cells, normalized to the mean for Bax+/+ mice, ± SEM.