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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Hepatology. 2012 Nov;56(5):1782–1791. doi: 10.1002/hep.25868

Fig. 4. Alb/AEG-1 hepatocytes are resistant to induction of senescence.

Fig. 4

A. Photomicrograph of WT and Alb/AEG-1 hepatocytes stained for senescence-associated β-galactosidase (SA-β-gal) after 1 week of culture. Graphical representation of quantification of SA-β-gal positive cells. B. Photomicrograph of WT and Alb/AEG-1 hepatocytes stained for γ-H2AX. Graphical representation of quantification of γ-H2AX foci/cell. For A and Bthe data represent mean ± SEM of three independent experiments. *: p<0.01. C. Analysis of expression level of the members of DNA damage response pathway in WT and Alb/AEG-1 hepatocytes by Western blotting. D. Total ROS level in WT and Alb/AEG-1 (TG) hepatocytes at day 1 and day 7 of culture. The data represent mean ± SEM of four independent experiments. *: p<0.01.