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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Hepatology. 2012 Nov;56(5):1782–1791. doi: 10.1002/hep.25868

Fig. 5. Conditioned media (CM) from Alb/AEG-1 hepatocytes induce angiogenesis.

Fig. 5

A. Human umbilical vein endothelial cells (HUVEC) were treated with CM from WT and Alb/AEG-1 hepatocytes and tube formation was photographed. Graphical representation of tube formation by HUVEC. The data represents mean ± SEM of three independent experiments. *: p<0.01. B. Chicken chorioallantoic membrane (CAM) was treated with CM from WT and Alb/AEG-1 hepatocytes and neovascularization was photographed. The numbers indicate percentage of new blood vessels arising from the existing blood vessels in naïve CAM when VEGF-treated CAM (used as positive control) was considered as 100%. The data represents mean ± SEM of three independent experiments. *: p<0.01. C. Confirmation of overexpression of Fibrinogen and FXII in CM from Alb/AEG-1 hepatocytes by Western blotting. Similar intensity of the non-specific bands indicate equal loading. The panels represent results from one representative set of mice which were confirmed in two additional sets.