Figure 1.
Impact of TLR9 deficiency on peanut-induced anaphylaxis and antibody and cytokine responses. TLR9 +/+ or −/− mice were orally sensitized to peanut with cholera toxin (PN/CT), followed by intraperitoneal challenge with crude peanut extract. (A) Body temperature measured 30 min after peanut challenge. Peanut (PN)-specific IgE (B), IgG1 (C) and IgA (D) as measured by ELISA in serum obtained 1-2 days prior to challenge. Cytokine secretion (E) measured after re-stimulation of spleen cells with peanut extract. Spleen cells were harvested immediately after allergen challenge. A is combined data from 2 independent experiments. B-E are from one representative experiment of 2, with n = 10 (+/+) and 8 (−/−). *** p < 0.001, ** p < 0.01, * p < 0.05 comparing the indicated groups.