Figure 1.

(a) Physical map of two heterochromatic genes, TPTE and TPTE2, and three euchromatic genes, STCH, SAMSN-1, and CAV2. (b) Replication timing in two controls (top) and in three ICF cell lines (bottom). x axis: time (h) of sample collection after the release in the S-phase; y axis: immunoprecipitated DNA divided by the corresponding input and represented as relative percentage of the last collected point (considered equal to 100%). Bars are means of 3–4 experiments with standard deviations. Plus and minus refer to gene expression in each cell line (data from ref. 12, except for CAV2, whose expression was analyzed in this work).