Figure 5. Rate constants for binding (k_on) and release (k_off) in site 1.

(a) Rate constants for Cy3-ATP. (b) Cy3-ADP. (c) Cy3-ATP with 10 mM Pi. (d) Cy3-ADP with 10 mM Pi. Rotation directions, long and short bindings, and [Cy3-nucleotide] are distinguished (bottom); identities of leaving nucleotides as explained in text. Shade associated with each curve indicates purely statistical errors: relative errors of n^−1/2 where n is the number of events observed per angular interval Δθ=10° (confidence level 68%; 95% at twice the shade width). Thick solid lines, the average with weights proportional to the total observation time (k_on) or the total occupied time (k_off).