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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2012 Sep 5;189(7):3311–3318. doi: 10.4049/jimmunol.1200597

Figure 4. Comparative costimulation of OT-II proliferation by TNFRSF4 and TNFRSF25 agonistic antibodies.

Figure 4

Adoptive transfer of OT-I and OT-II cells was performed as described in figure 1, following by immunization with either 3T3-ova-gp96-Ig (A) or ova/alum (B) together with the indicated antibodies as described in figure 2. OT-II proliferation in the peripheral blood was measured by flow cytometry on the indicated days, and representative plots of Vα2+Vβ5+ cells (pre-gated on CD3+CD4+ cells) are illustrated in (C) on day 5 for ova/alum immunized mice. Data indicate mean ± SEM for ≥ 5 mice in each of ≥ 2 independent experiments.