. Author manuscript; available in PMC: 2013 Oct 1.

Published in final edited form as: J Immunol. 2012 Sep 5;189(7):3311–3318. doi: 10.4049/jimmunol.1200597

Table 1.

Summary of OT-I, OT-II and Treg responses to immunization with adjuvanted (alum) versus cross-presented (gp96-Ig) ovalbumin with and without costimulation by agonistic antibodies to TNFRSF4 (OX86) and TNFRSF25 (4C12).

Vaccination Method	CD4+ Response	CD8+ Response	Treg Response
Ova/alum	+	+	−
Ova/alum + OX86	++	++	+
Ova/alum + 4C12	+	++	++
Ova/alum+OX86+4C12	++	+++	+++
3T3-ova-gp96-Ig	−	+	−
3T3-ova-gp96-Ig + OX86	−	++	+
3T3-ova-gp96-Ig+ 4C12	−	++	++
3T3-ova-gp96-Ig + OX86 + 4C12	−	+++	+++