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Kinetics of the inactivation of β-galactosidase in the absence and presence of 3 M GdnHCl at 50°C, pH 4.5, and at different concentrations of substrate, PNPG, [0.10–0.60]. (a) In the absence of GdnHCl (no GdnHCl); (b) in the presence of 3 M GdnHCl; (c) in the presence of 3 M GdnHCl and 5 mM galactose; (d) in the presence of 3 M GdnHCl and 10 mM galactose; (e) in the presence of 3 M GdnHCl and 20 mM galactose.
