Table 1.

Effect of uracil derivatives or P2 receptors antagonists on cardiomyocytes subjected to hypoxia

Treatment	LDH release (%)
Normoxia	4.2 ± 0.6
Hyp	40 ± 2.4
Hyp + UTP	10 ± 1.6*
Hyp + uridine	29 ± 2.5
Hyp + UDP	36 ± 2.6
Hyp + 5-HD	37 ± 0.6
Hyp + UTP + 5-HD	12 ± 1.6*
Hyp + UTP + PPADS	32 ± 3.6
Hyp + UTP + suramin	25 ± 3.5
Hyp + UTP + RB-2	11 ± 0.8*

Cardiomyocytes were treated with 50 μM uridine, UDP, or UTP or with P2 receptor antagonist PPADS (10 μM), RB-2 (50 μM), or suramin (300 μM) or with 5-HD, a mitochondrial K_ATP channel inhibitor (300 μM), for 15 min before hypoxia. The cells were then washed twice and subjected to hypoxia for 2 h in glucose-free PBS, at 37 °C. The amount of LDH released to the medium was determined and compared to the total activity of control homogenate (100%). Data are means of at least three replicates in three separate experiments ± S.D.

^*P < 0.01 compared with hypoxia.