Table 2.
ELISA of A2A Receptor Mutants on the Surface of COS-7 Cells
mutant | expression levela (%) |
---|---|
V84A | 98.2 ± 4.0 |
V84D | 99.4 ± 6.7 |
H278N | 95.7 ± 5.6 |
H278K | 97.5 ± 4.4 |
Table shows expression level as a percentage of HA-tagged A2A wild type (100%), n = 8. Expression level was determined using a standard curve (Kim et al., 1995). None of the values listed were significantly different from control.