Figure 2.
(a) Galactose, lactose, and aminopropyl galactose (gal-NH2) were probed with 400 ng/mL RCA120-HRP; glycan bioactivity was observed on the DVS+ membrane but not on the DVS− membrane. There was no signal from the mannose and control (no glycan functionalized) spots, indicating that non-specific binding of RCA120 was negligible. (b) Maltose, glucose, RNase B, and mannose were bound by Con A-HRP on the DVS+ membrane but not the DVS− membrane. There was little signal from the galactose and control spots, indicating that non-specific binding of Con A was negligible. Representative colorimetric spots from the membranes appear below each corresponding bar (data were averaged over 6 spots for each carbohydrate).