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Expression of mRNA for IL-12 and IFN-γ production after exogenous addition of PPD plus IL-12. mRNA for IL-12 was determined by RT-PCR in unstimulated cultures of PBMC from seronegative healthy subjects, co-infected patients, and patients with tuberculosis only (A). IFN-γ production was evaluated by ELISA in supernatants of PBMC cultures unstimulated or stimulated with PPD and PPD plus IL-12 in the concentration of 50 μg/ml (B and C).
