Figure 1.
a) Core-satellite Au nanoassemblies before and after proteolytic cleavage. b) Immobilization of the negatively charged AuNP cores is enabled by Coulombic electrostatic attraction to an APTES monolayer on glass. The glass slide is subsequently passivated with an mPEG chain to block nonspecific satellite adsorption. c) Satellite attachment is enabled by a directional cysteine/biotin-streptavidin peptide tether. d) The addition of trypsin cleaves the peptide, releasing the satellites into solution and enabling colorimetric detection of the protease.