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. 2012 Aug 2;137(1):37–47. doi: 10.1111/j.1365-2567.2012.03607.x

Figure 4.

Figure 4

Lung natural killer (NK) cells express higher levels of inhibitory receptors and lower levels of activating receptors. Lymphocytes were isolated from bone marrow (BM), spleen and lung in normal C57BL/6 mice. (a) Flow cytometry assay determined the expression of inhibitory receptors (CD94 and NKG2A) on NK cells (CD3− NK1.1+), and the frequencies are shown as mean ± SEM. (b) Flow cytometry assay determined the expression of activating receptors (NKp46 and NKG2D) on NK cells, and the frequencies are shown as mean ± SEM. (c) Flow cytometry assay determined the expression of CD69 and CD244 on NK cells, and the mean fluorescence intensity (MFI) values are shown as mean ± SEM. Histograms are representative of three independent experiments using three mice per group, the shadow histogram is the isotype control. *P < 0·05; **P < 0·01; ***P < 0·001; NS, not significant.