Figure 7.

An experimental trace (A) and the results from several experiments (B) showing how flow-induced dilatation varied with flow rate (0.5–5 mL·min⁻¹ for 2 min) in carotid artery segments at ∼75% of maximal U46619-induced tone. Dilatation was unaffected by combined treatment with L-NAME (100 µM) and indomethacin (Indo, 3 µM), but was inhibited by the combination of apamin (Ap, 0.5 µM) and TRAM-34 (10 µM) with indomethacin. Inhibition by the latter combination was enhanced following additional inclusion of L-NAME. The 2 min periods of flow are indicated by the time bars. The pressure trace (in mmHg) shows that flow was induced while maintaining constant pressure. Data are presented as the mean ± SEM of four or five experiments. **P < 0.01 and ***P < 0.001 indicate significant differences from respective controls. ^#P < 0.05 and ^##P < 0.01 indicate a significant difference from tissues treated with the combination of apamin, TRAM-34 and indomethacin.