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. 2012 Oct;167(3):612–626. doi: 10.1111/j.1476-5381.2012.02040.x

Figure 6.

Figure 6

LeTx (100 ng·mL−1 for 3 h)-induced autophagy in H9C2 cells in the absence or presence of the lysosomal inhibitor pepstatin-A. (A) Representative microscopic images of GFP-LC3II (puncta shown in arrowheads). (B) Quantitation of cells with autophagosomes. (C) LC3-II/LC3-I ratio. Inset: Representative gel blots depicting expression of LC3 and GAPDH (loading control). Mean ± SEM, n= 3 independent cultures, *P < 0.05 versus control group, #P < 0.05 versus LeTx group.