Table 1.

Estimated pEC₅₀, E_max and Hill coefficients (n_H)

Drug	Pre-treatment (h)	pEC50	Emax	nH
Gemfibrozil	24	5.9 ± 1.7	16.9 ± 3.5	1.4 ± 0.4
	72	6.2 ± 0.1	41.7 ± 4.5	1.0 ± 0.1
GW0742	24	8.0 ± 0.1	18.8 ± 1.4	1.0 ± 0.2
	72	9.0 ± 0.1	36.4 ± 1.4	1.1 ± 0.2
Pioglitazone	24	7.0 ± 0.2	31.3 ± 2.2	1.7 ± 0.1
	72	7.9 ± 0.1	58.2 ± 2.8	1.1 ± 0.2

Immortalized podocytes were pre-treated with gemfibrozil (0.01–30 µM), GW0742 (0.1 nM to 0.1 µM) or pioglitazone (1 nM to 1 µM) for either 24 h (a single treatment) or 72 h (as repeated treatments), then exposed to ND (5 h). At the end of the recovery phase (24 h), the number of viable cells was assessed to determine the ND-induced cell loss. Results are expressed as mean ± SEM. Mean of four experiments run in triplicate. Data were fitted as sigmoidal concentration–response curves and analysed with a four-parameter logistic equation.