Figure 7.

Effects of ACh on endothelial [Ca²⁺]_i in rabbit jugular vein. (A) [Ca²⁺]_i as estimated from the Fura-2 ratio. Application of ACh (3 µM) for 2 min significantly increased [Ca²⁺]_i. Data are shown as mean ± SEM. *P < 0.05 vs. ‘Basal’. (B) Concentration-dependent effect of ACh (10⁻⁸–3 × 10⁻⁶ M) on [Ca²⁺]_i[shown as Δ Fura-2 ratio (F₃₄₀/F₃₈₀)] in endothelial cells. Data are shown as mean ± SEM (n= 6–9). (C). Effect of TRAM 34 (10 µM) on ACh (1 µM)-induced increase in [Ca²⁺]_i[shown as Δ Fura-2 ratio (F₃₄₀/F₃₈₀)] in endothelial cells. Data are shown as mean ± SEM (n= 6).