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. Author manuscript; available in PMC: 2013 Sep 14.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Aug 19;426(1):148–152. doi: 10.1016/j.bbrc.2012.08.054

Figure 4. Stimulation of tyrosine hydroxylase expression and norepinephrine generation by ascorbate.

Figure 4

Panels A and B: SH-SY5Y cells were treated in culture with the indicated concentration of ascorbate for 24 h, rinsed twice in KRH, and removed from the plate for Western blotting as described in Methods. Panel A shows a representative Western blot with measured intracellular ascorbate concentrations in a parallel cell incubation. Panel B shows Western blots from 4 separate experiments, normalized for gel protein loading to β-actin. “TH” = tyrosine hydroxylase. An “*” indicates p < 0.05 compared to zero ascorbate by repeated measures one-way ANOVA and Dunn’s test. Panel C: Cells were treated for 24 h in culture with the indicated concentration of ascorbate in the absence or presence of 5 µg/ml cycloheximide, as indicated. Results are shown from 4 experiments with an asterisk (*) indicating p < 0.05 compared to cells not treated with cycloheximide by two-way ANOVA and the Bonferroni test.