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. Author manuscript; available in PMC: 2012 Sep 24.
Published in final edited form as: Biomed Pharmacother. 2009 Jun 9;63(6):429–435. doi: 10.1016/j.biopha.2009.04.045

Fig. 1.

Fig. 1

TNF-α elevates AP-1 luciferase reporter activity. MCF7 cells were transiently cotransfected with the firefly luciferase reporter construct AP-1-LUC and β-galactosidase plasmid (as an internal control) plus pCMV-TAM-67 or pCMV-neo vector. Eight hours after transfection, cells were serum starved for 24 h, and then treated with TNF-α (20 ng/ml) or vehicle (Control) for the indicated periods of time. Then cells were harvested for luciferase reporter assays. The relative values of AP-1 luciferase activity to β-galactosidase are shown as means ± SEM of three independent experiments. *P < 0.05 vs. levels in cells cotransfected with pCMV-neo vector and treated with vehicle.