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. Author manuscript; available in PMC: 2012 Sep 24.
Published in final edited form as: Biomed Pharmacother. 2009 Jun 9;63(6):429–435. doi: 10.1016/j.biopha.2009.04.045

Fig. 3.

Fig. 3

AP-1 complex is composed of p-c-Jun-c-Jun and p-c-Jun-JunB homodimers after TNF-α treatment. MCF7 cells were serum starved for 24 h and treated without (Control) or with TNF-α (20 ng/ml) for 3 h or 24 h. Cell nuclear lysates were subjected to immunoprecipitation (IP) with an antibody against p-c-Jun followed by immunoblotting (IB) with antibodies against c-Jun, c-Fos and JunB. Representative Immunoblots of three separate experiments are shown.