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. Author manuscript; available in PMC: 2012 Sep 24.
Published in final edited form as: Biomed Pharmacother. 2009 Jun 9;63(6):429–435. doi: 10.1016/j.biopha.2009.04.045

Fig. 4.

Fig. 4

Effects of TNF-α on the activities of MAPK pathways. MCF7 cells were serum starved for 24 h and treated with TNF-α (20 ng/ml) for the indicated periods of time, and JNK (p-JNK), P38 (p-p38) and ERK (p-ERK) activation was assessed using phosphorylation specific antibodies. The levels of β-actin protein in each group were used as the references. Representative immunoblots of three separate experiments are shown.