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. Author manuscript; available in PMC: 2012 Sep 24.
Published in final edited form as: Biomed Pharmacother. 2009 Jun 9;63(6):429–435. doi: 10.1016/j.biopha.2009.04.045

Fig. 6.

Fig. 6

TNF-α stimulates VEGF mRNA transcription in MCF7 cells. MCF7 cells were treated with 20 ng/ml of TNF-α for the indicated time periods after 24 h serum starvation. Total RNA was isolated and 1 µg RNA was used for RT-PCR analyses of VEGF and GAPDH (as a reference) mRNA levels. PCR products were revealed by ethidium bromide staining under UV after agarose gel electrophoresis. Representative graphs of three separate experiments are shown.