Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
TNF-α increases VEGF protein levels in MCF7 cells. After serum starvation for 24 h, MCF7 cells were pretreated without or with SP600125 for 30 min, and treated with TNF-α (20 ng/ml) for the indicated time periods. Cell lysates were collected for Western blot analyses with antibodies against VEGF or β-actin (as a loading control). Representative Immunoblots of three separate experiments are shown.
