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. Author manuscript; available in PMC: 2012 Sep 24.
Published in final edited form as: Biomed Pharmacother. 2009 Jun 9;63(6):429–435. doi: 10.1016/j.biopha.2009.04.045

Fig. 8.

Fig. 8

p-c-Jun is recruited to the VEGF promoter following TNF-α treatment. After serum starvation for 24 h, MCF7 cells were treated without (Control) or with TNF-α (20 ng/ml) for the indicated time periods, and chromatin protein-DNA complexes were cross-linked using formaldehyde. The purified nucleoprotein complexes were immunoprecipitated with Ser73-phosphorylated-c-Jun antibody (p-c-Jun) or nonimmune IgG and DNA samples recovered were amplified by PCR as detailed in Materials and Methods. Representative graphs of three separate experiments are shown.