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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):3043–3052. doi: 10.1002/jcp.22652

Figure 3. Putative C/EBP site II is a key component in the C/EBPβ-enhanced Runx2 P1 promoter activity.

Figure 3

Transient co-transfection experiments were performed in C2C12 and MC3T3 cells (left and right panels, respectively), using luciferase reporter constructs carrying different deletions of the rat Runx2 P1 promoter (500 ηg) and increasing amounts of the pC/EBPβ expression plasmid. A) 600 bp rat Runx2 P1 promoter construct, B) 490 bp Runx2 P1 promoter construct, C) 288 bp Runx2 P1 promoter construct, and D) 108 bp Runx2 P1 promoter construct. The presence (+) or absence (−) of each construct in the transfection mix is indicated below the bars. These results represent at least three independent experiments, each assayed in triplicate. Each bar represents the mean +/− standard error. Statistical significance was determined by the ANOVA test (*: p<0.05; **: p<0.01).