Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):3043–3052. doi: 10.1002/jcp.22652

Figure 5. C/EBP site II is a principal component in the C/EBPβ-mediated transcriptional up-regulation of the Runx2 P1 promoter.

Figure 5

A) Schematic representation of the wild type and mutated versions of the 288P1rRunx2-Luc reporter constructs. The figure shows the sequences of the putative C/EBP sites I and II present in these reporter constructs. Nucleotides marked with an asterisk were replaced by the nucleotides showed in lowercase letters. B) C2C12 and MC3T3 cells (upper and lower graphs, respectively) were transiently transfected with 250 ηg of the reporter constructs containing intact putative C/EBP sites I and II (p288P1rRunx2-Luc), a mutated C/EBP site I (p288P1rRunx2MUT1-Luc), a mutated C/EBP site II (p288P1rRunx2MUT2-Luc), or both C/EBP sites I and II mutated (p288P1rRunx2MUT1+2-Luc). Cells were also co-transfected with a vector for C/EBPβ over-expression (pC/EBPβ) in the amounts indicated under each graph. These results represent at least three independent experiments, each assayed in triplicate. Each bar represents the mean +/− standard error. Statistical significance was determined by the ANOVA test (*: p<0.05; **: p<0.01).