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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Cell Physiol. 2011 Nov;226(11):3043–3052. doi: 10.1002/jcp.22652

Figure 6. C/EBPβ requires the putative C/EBP site II to up-regulate the Runx2 P1 promoter activity in Runx2-null mice osteoprogenitor cells.

Figure 6

A) TERT-immortalized mouse Runx2-null osteoprogenitor cells were co-transfected with 250 ηg of the wild type or mutant versions of the p288P1rRunx2-Luc reporter construct and increasing amounts of the C/EBPβ expression vector (pC/EBPβ). Each bar represents the mean +/− standard error. Statistical significance was determined by the ANOVA test (*: p<0.05; **: p<0.01; N.S. not statistically significant). B) Western blot analysis of the mouse Runx2-null cells transfected with the pC/EBPβ vector. Nuclear extracts (10 μg) were fractionated by SDS-PAGE (12% acrylamide) and revealed by Western blot using specific antibodies against C/EBPβ or the Xpress-tag to detect the over-expressed Xpress-tagged C/EBPβ/LAP* (o.e.). Detection of TFIIB was used to control for equal protein loading.