Fig. 1. Experimental animal model.
Juvenile mice (21–25 days old) were given a single (i.p.) injection of vehicle (olive oil) or DEN (1 mg/kg). Silibinin diet was introduced at 15 or 39 weeks, continuing for 9 weeks with or without ethanol drinking-water initiation at 16 or 40 weeks. Animals were sacrificed at 24 or 48 weeks.