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. Author manuscript; available in PMC: 2013 Dec 29.
Published in final edited form as: Cancer Lett. 2012 Aug 1;326(1):88–95. doi: 10.1016/j.canlet.2012.07.028

Fig. 4. Silibinin feeding did not significantly inhibit cytochrome P4502E1 or alter hepatic oxidative stress status in DEN-initiated male and female mice.

Fig. 4

(a) Representative immunoblots for detection of CYP2E1 protein in control and DEN-initiated animals fed silibinin alone or in combination with ethanol. Equal loading was verified by b-actin expression (data not shown). (b) Quantitative analysis of the effect of silibinin on CYP2E1 activity. (c) malondialdehyde (MDA) and (d) glutathione (GSH) levels were measured 48 weeks post-DEN initiation in male and female mice fed dietary silibinin with or without ethanol in the drinking water. Data are presented as mean ± SEM. (S; dietary silibinin, D; DEN-initiated, DS; DEN-initiated/dietary silibinin, DES; DEN-initiated/dietary silibinin/EtOH). n≥5 animals/group, p<.05 S vs D, DS and DES in male and female groups, # male vs female, § DES vs D and DS, † DS vs D.