Fig. 5. Silibinin feeding evoked differential immunological effects in male and female mice in the setting of HCC alone and concomitant with ethanol feeding.
(a,b,c,d) Relative expression of IL6, Tbet, Gata3 and SMAD3 mRNA in total liver as assessed by qRT-PCR. Data are presented as mean ± SEM. (S; dietary silibinin, D; DEN-initiated, DS; DEN-initiated/dietary silibinin, DES; DEN-initiated/dietary silibinin/EtOH). n≥5 animals/group, p<.05 S vs D, DS and DES in male and female groups (a, c and d; b – female groups only), # male vs female, § DES vs D and DS, † DS vs D.