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. 2012 Sep 24;6:65. doi: 10.3389/fncir.2012.00065

Figure 2.

Figure 2

Neurogenesis period of cortical nNOS cells. (A) nNOS-positive neurons and EdU-incorporating cells at P30 following EdU administration at different developmental stages. White arrows and arrowheads indicate nNOS-immunoreactive neurons colabeled with EdU. These are shown at higher magnification in the lower panel for type I (arrows) and type II cells (arrowheads). (B) Histogram showing the EdU labeling index for nNOS type I cells. (C) Neurogenesis periods of nNOS type I cells residing in lower and upper cortical layers. A significant difference is detected at E12. **P < 0.01 (Two-way ANOVA with Bonferroni post-hoc test). (D) Histogram showing the EdU labeling index for nNOS type II cells. (E) Neurogenesis periods of nNOS type II cells residing in lower and upper cortical layers. A significant difference is detected at E12, E14, and E16. **P < 0.01 ***P < 0.001 (Two-Way ANOVA with Bonferroni post-hoc test). Error bars indicate SEM. Scale bars: A, upper panel, 100 μm, lower panel, 20 μm.