Table II.
Protein sequences and RP-HPLC retention times
| Name | Sequence | tR (min) |
|---|---|---|
| A | ASVSDVPRDLEVVAATPTSLLISWFDYAVTYYRITYGETGGNSPVQEFTV PGWISTATISGLNPGVDYTITVYAVTDNSHWPFRSTPISINYRTGSHHHHHH | 21.2 |
| A-phil | ASSSDSPRDLEVTNATPNSLTISWFDYAVTYYRITYGETGGNSPSQEFTV PGWISTATISGLNPGQDYTITVYAVTDNSHWPFRSTPISINYRTGSHHHHHH | 18.0 |
| D | VSDVPRDLEVVAATPTSLLISWLHHRSDVRSYRITYGETGGNSPVQEFTV PGSRSLATISGLRPGVDYTITVYAVTWGSYCCSNPISINYRTHHHHHH | 17.8 |
| D-phil | ASSSDSPRDLEVTNATPNSLTISWLHHRSDVRSYRITYGETGGNSPSQEFTV PGSRSLATISGLRPGQDYTITVYAVTWGSYCCSNPISINYRTGSHHHHHH | 15.7 |
Mutations from the parental protein are boldfaced and underlined. tR denotes retention time for RP-HPLC on analytical C18 column with a gradient of 10–90% acetonitrile in water with 0.1% trifluoroacetic acid from 2 to 32 min.