Table 2.

Medium composition used in perfusion microcarrier culture of Vero cells

Component	Volume (mL per L)
10X DMEM*	100
NaHCO3 (7.5%)	49.3
Glutamine (200 mM)	20
Nystatin (10,000 units per mL)	24
Penicillin-Streptomycin Solution	10
Newborn Calf Serum	10
Folic Acid Solution (0.4% w/v)	1
Gas Vesicle Suspension (2.3% v/v) or Distilled Water	785.7

* Dulbecco’s Modified Eagle’s Medium (DMEM), with 4 g L⁻¹ of glucose