Linker length has large effects on the stability
of single-chain Arc to urea denaturation. The sequences of linkers LL9
(○), LL11 (Δ), LL17 (•), LL19 (□), LL31
(▴), and LL47 (▪) are listed in Fig. 3A.
Fraction unfolded was calculated by fitting plots of CD ellipticity
(234 nm) vs. urea concentration to a two-state-unfolding transition.
The solid lines represent the best theoretical fits of the experimental
data.